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. 1976 Jan;51(1):7-11.
doi: 10.3109/10520297609116662.

The use of tannic acid-glutaraldehyde in the study of elastic and elastic-related fibers

G Cotta-Pereira, F G Rodrigo, J F David-Ferreira

The use of tannic acid-glutaraldehyde in the study of elastic and elastic-related fibers

G Cotta-Pereira et al. Stain Technol. 1976 Jan.

Abstract

Fixation with tannic acid-glutaraldehyde permits distinction of oxytalan, elaunin and elastic fibers in the electron microscope. The results obtained using tannic acid at concentrations of 1.0%, 0.5% and 0.25% in 3% glutaraldehyde were compared. The 0.25% concentration is recommended for studying fine details of connective fibrils and for regular staining of elastin.

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