Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity
- PMID: 594188
Item in Clipboard
Studies on a rat liver subcellular poly A-rich RNA fraction with glutamate dehydrogenase template activity
Physiol Chem Phys.
1977.
Abstract
A heterogeneous poly A-mRNA fraction was isolated from rat liver microsomes by phenol:chloroform extraction, millipore filtration, and poly U-agarose affinity chromatography. The fractions were characterized by their secondary structures and poly A contents. From translational studies, the isolated fraction was found to have high glutamate dehydrogenase template activity in cell-free systems containing microsomes or polysomes. A spectrophotometric procedure for following enzyme biosynthesis was also developed.
Similar articles
-
Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.Mol Biol Rep. 1986;11(3):131-5. doi: 10.1007/BF00419732. Mol Biol Rep. 1986. PMID: 2876375
-
Translation of mRNA for glutamate dehydrogenase and spectrophotometric procedure to follow the enzyme biosynthesis.Arch Int Physiol Biochim. 1977 Aug;85(3):583-8. doi: 10.3109/13813457709069874. Arch Int Physiol Biochim. 1977. PMID: 72534
-
Translation of glutamate dehydrogenase mRNA in a reticulocyte cell-free system.Physiol Chem Phys. 1979;11(1):71-5. Physiol Chem Phys. 1979. PMID: 504364
-
[Isolation and study of polyadenylated RNA from Bacillus intermedius].Mikrobiologiia. 1988 May-Jun;57(3):421-5. Mikrobiologiia. 1988. PMID: 2460725 Russian.
-
Antigen-specific messenger RNA for immunological research: purification and characterization of RNA from intestinal epithelial cells using specific antibody.J Clin Lab Immunol. 1987 Feb;22(2):49-58. J Clin Lab Immunol. 1987. PMID: 3298654
Cited by
-
Nuclear deprimerones (low molecular weight peptides controlling gene expression) are associated with DNA and nuclear RNA.Mol Biol Rep. 1982 Apr 16;8(3):157-65. doi: 10.1007/BF00777243. Mol Biol Rep. 1982. PMID: 6181390
-
Glutamate dehydrogenase activity in subcellular fractions of mouse fibrosarcoma.Mol Biol Rep. 1979 Dec 31;5(4):251-2. doi: 10.1007/BF00782898. Mol Biol Rep. 1979. PMID: 530274
-
Identification of messenger RNA for glutamate dehydrogenase using a spectrophotometric probe.Mol Biol Rep. 1986;11(3):131-5. doi: 10.1007/BF00419732. Mol Biol Rep. 1986. PMID: 2876375