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. 1977 Nov;14(5):813-7.
doi: 10.1016/0090-6980(77)90298-2.

Prostaglandin and thromboxane production by rat decidual microsomes

K I Williams, I Downing

Prostaglandin and thromboxane production by rat decidual microsomes

K I Williams et al. Prostaglandins. 1977 Nov.

Abstract

The preparation of a microsomal fraction from the decidual tissue of pregnant rat uteri is described. Incubation of such microsomes with a mixture of radiolabelled and cold arachidonic acid (51 micrometer) plus cofactors resulted in a 30% substrate conversion. Products were resolved into four peaks (A, B, C and D) by thin-layer chromatography. Combined gas-liquid chromatography-mass spectrometry and further thin-layer chromatography identified the products as PGF2 alpha (A); thromboxane B2 (B); a mixture of 6-OXO PGF1 alpha and PGE2 (C); PGD2 and PGE2 (D). PGE2 was the major product.

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