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. 1966 Mar;7(2):324-7.

Complete separation of lipid classes on a single thin-layer plate

C P Freeman, D West
  • PMID: 5947043
Free article

Complete separation of lipid classes on a single thin-layer plate

C P Freeman et al. J Lipid Res. 1966 Mar.
Free article

Abstract

A double-development procedure employing first a polar and then a nonpolar solvent system is described for the complete separation by thin-layer chromatography of the main lipid classes encountered in natural lipids. For better quantification, long plates (34 cm) are employed. Diglycerides were separated from cholesterol, 1,2- from 1,3-diglycerides, and monoglycerides from phospholipids.

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