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Comparative Study
. 1977;18(4):509-14.
doi: 10.1186/BF03548414.

Comparative grouping of mastitis Streptococci by means of co-agglutination and precipitation

F Saxegaard
Comparative Study

Comparative grouping of mastitis Streptococci by means of co-agglutination and precipitation

F Saxegaard. Acta Vet Scand. 1977.

Abstract
in English, German

Two hundred bovine mastitis streptococcal strains belonging to groups B, C, E, G and L were tested comparatively by means of the co-agglutination and precipitation technique. Identical results were obtained with the two tests in 191, or 95.5 %, of the strains. Six strains, which could not be grouped by co-agglutination, proved to belong to group B when grouped by precipitation. On the other hand, one strain which proved to belong to group G and two strains to group L when grouped by co-agglutination, could not be grouped by precipitation. Some cross-reactivity was observed between groups A and C, B and G, B and L. Only a few L strains showed marked cross-reactivity which was not easily distinguished from specific reactions. However, the cross-reactivity ought to be eliminated by dilution or adsorption. Using the precipitation test as a supplementary method, the easy and rapid co-agglutination test was found to be a suitable procedure for routine grouping of mastitis streptococci.

To hundre streptokokkstammer isolert fra tilfelle av mastitt hos storfe ble gruppet ved hjelp av koagglutinasjons- og presipitasjons-metoden og viste seg å tilhøre Lancefields gruppe B, C, E, G og L. Identiske resultater med de 2 metoder ble oppnådd med 191 eller 95,5 % av stammene. Seks stammer som ved presipitasjon viste seg å tilhøre gruppe B, lot seg ikke gruppe ved koagglutinasjon. En stamme som ved koagglutinasjon viste seg å tilhøre gruppe G og 2 som viste seg å tilhøre gruppe L, lot seg ikke gruppe ved presipitasjon. En viss kryssreaktivitet ble observert henholdsvis mellom gruppe A og C, gruppe B og G, gruppe B og L. Bortsett fra noen få L stammer var kryssreaktiviteten som bør kunne elimineres ved fortynning eller adsorpsjon av sera, ikke mer uttalt enn at den lett kunne adskilles fra spesifikke reaksjoner. Med bruk av presipitasjonstesten som supplerende test ble den enkle og hurtige koagglutinasjonstesten funnet å være vel egnet som rutinetest ved grupping av mastittstreptokokker.

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