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. 1966 Jul;100(1):69-72.
doi: 10.1042/bj1000069.

The antigen-binding capacity of the peptide chains of horse antibodies

R C Weir, R R Porter

The antigen-binding capacity of the peptide chains of horse antibodies

R C Weir et al. Biochem J. 1966 Jul.

Abstract

The antigen-binding capacity of the peptide chains of horse anti-(diphtheria toxin) has been studied by using (125)I-labelled toxoid and electrophoresis of antibody-antigen mixtures on cellulose acetate. The heavy chains retained about 20% of the activity of the whole antibody and the light chains less than 5%. Recombination of specific heavy and light chains gave about 60% recovery of activity and recombination of specific heavy chains and non-specific light chains about 40% recovery. It is suggested that these results favour the heavy chain as the major site of the antigen-binding activity.

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