Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1966 Aug;100(2):479-84.
doi: 10.1042/bj1000479.

A radiochemical method for the estimation of choline acetyltransferase

F Fonnum

A radiochemical method for the estimation of choline acetyltransferase

F Fonnum. Biochem J. 1966 Aug.

Abstract

1. A radiochemical method for the estimation of choline acetyltransferase (choline acetylase) has been devised which involves the formation of labelled acetylcholine from labelled acetate. 2. [1-(14)C]Acetate and coenzyme A are pre-incubated in the presence of non-rate-limiting concentrations of acetyl-coenzyme A synthetase to give [1-(14)C]acetyl-coenzyme A, which then reacts with choline in the presence of the acetyltransferase to give [(14)C]acetylcholine. 3. Any [(14)C]-acetyl-coenzyme A remaining at the end of the reaction is destroyed by the addition of excess of hydroxylamine, and [(14)C]acetylcholine is freed from other labelled compounds by precipitation with sodium tetraphenylborate (Kalignost). 4. The washed precipitate is dissolved in acetonitrile-benzyl alcohol and estimated by scintillation counting. 5. Advantages over other methods are discussed.

PubMed Disclaimer

References

    1. Experientia. 1964 Dec 15;20(12):677-8 - PubMed
    1. J Neurochem. 1965 Apr;12:253-9 - PubMed
    1. Acta Physiol Scand. 1954 Nov;32(2-3):163-73 - PubMed
    1. J Physiol. 1956 Nov 28;134(2):385-92 - PubMed
    1. Biochem J. 1959 Nov;73:447-58 - PubMed