Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1966 Sep;100(3):827-32.
doi: 10.1042/bj1000827.

Leucine aminopeptidase in extracts of swine muscle

R L Joseph, W J Sanders

Leucine aminopeptidase in extracts of swine muscle

R L Joseph et al. Biochem J. 1966 Sep.

Abstract

1. Leucine aminopeptidase (EC 3.4.1.1) has been demonstrated in swine muscle at a level of activity one-fifth that of the swine kidney. 2. The enzyme has been purified 110-fold by precipitation with ammonium sulphate, heat treatment and chromatography on Sephadex G-100. 3. The enzyme is heat-stable, but is rapidly inactivated below pH7. It requires Mg(2+) or Mn(2+) for activity. The Michaelis constant for leucine amide with Mg(2+)-activated enzyme is 5.0x10(-3)m. 4. Muscle leucine aminopeptidase is very similar to the kidney enzyme.

PubMed Disclaimer

References

    1. Biochem J. 1964 Mar;90(3):509-12 - PubMed
    1. Biochem J. 1964 Mar;90(3):513-8 - PubMed
    1. J Biol Chem. 1950 Nov;187(1):127-35 - PubMed
    1. Adv Protein Chem. 1961;16:197-219 - PubMed
    1. J Biol Chem. 1955 Jan;212(1):271-99 - PubMed