Leucine aminopeptidase in extracts of swine muscle
- PMID: 5969296
- PMCID: PMC1265222
- DOI: 10.1042/bj1000827
Leucine aminopeptidase in extracts of swine muscle
Abstract
1. Leucine aminopeptidase (EC 3.4.1.1) has been demonstrated in swine muscle at a level of activity one-fifth that of the swine kidney. 2. The enzyme has been purified 110-fold by precipitation with ammonium sulphate, heat treatment and chromatography on Sephadex G-100. 3. The enzyme is heat-stable, but is rapidly inactivated below pH7. It requires Mg(2+) or Mn(2+) for activity. The Michaelis constant for leucine amide with Mg(2+)-activated enzyme is 5.0x10(-3)m. 4. Muscle leucine aminopeptidase is very similar to the kidney enzyme.
Similar articles
-
Purification and enzymatic properties of an L-leucine aminopeptidase from swine liver.Biochim Biophys Acta. 1981 Aug 13;660(2):262-70. doi: 10.1016/0005-2744(81)90169-8. Biochim Biophys Acta. 1981. PMID: 7284403
-
Preparation of leucine aminopeptidase free of endopeptidase activity.Biochemistry. 1968 Jun;7(6):2085-93. doi: 10.1021/bi00846a010. Biochemistry. 1968. PMID: 5660039 No abstract available.
-
Human liver L-leucine aminopeptidase: evidence for two forms compared to pig liver enzyme.Biochimie. 1983 Jul;65(7):397-404. doi: 10.1016/s0300-9084(83)80059-5. Biochimie. 1983. PMID: 6414529
-
[Aminopeptidases, amino acid arylamidases and neuraminidase as constituents of a catabolic enzyme system in the corpus luteum].Fortschr Geburtshilfe Gynakol. 1973;50:101-71. Fortschr Geburtshilfe Gynakol. 1973. PMID: 4585595 Review. German. No abstract available.
-
Leucine aminopeptidase activity by flow cytometry.Methods Cell Biol. 1994;41:461-7. doi: 10.1016/s0091-679x(08)61735-5. Methods Cell Biol. 1994. PMID: 7861976 Review. No abstract available.
Cited by
-
Separation of rat muscle aminopeptidases.Biochem J. 1976 May 1;155(2):375-81. doi: 10.1042/bj1550375. Biochem J. 1976. PMID: 938487 Free PMC article.
-
Properties and activities of aminopeptidases in normal and mitogen-stimulated human lymphocytes.Biochem J. 1985 Feb 15;226(1):59-65. doi: 10.1042/bj2260059. Biochem J. 1985. PMID: 3977881 Free PMC article.
-
Characterization of the protease activity in the chemotactic factor inactivator.J Clin Invest. 1976 Jul;58(1):123-9. doi: 10.1172/JCI108440. J Clin Invest. 1976. PMID: 6489 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources