Photosynthesis by sugar-cane leaves. A new carboxylation reaction and the pathway of sugar formation

Abstract

1. Radioactive products in detached leaf segments were examined after periods of steady-state photosynthesis in (14)CO(2). 2. After exposure to (14)CO(2) for approx. 1sec. more than 93% of the fixed radioactivity was located in malate, aspartate and oxaloacetate. After longer periods large proportions of the radioactivity appeared in 3-phosphoglycerate, hexose monophosphates and sucrose. Similar results were obtained with leaves still attached to the plant. 3. Radioactivity appeared first in C-4 of the dicarboxylic acids and C-1 of 3-phosphoglycerate. The labelling pattern in hexoses was consistent with their formation from 3-phosphoglycerate. 4. The reaction giving rise to C(4) dicarboxylic acid appears to be the only quantitatively significant carboxylation reaction. 5. Evidence is provided that the radioactivity incorporated into the C(4) dicarboxylic acid pool is transferred to sugars via 3-phosphoglycerate. A scheme is proposed to account for these observations.