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. 1976 Jul;73(7):2191-5.
doi: 10.1073/pnas.73.7.2191.

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities

D L Kacian, J C Myers

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities

D L Kacian et al. Proc Natl Acad Sci U S A. 1976 Jul.

Abstract

The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.

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References

    1. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2555-8 - PubMed
    1. J Mol Biol. 1975 May 25;94(3):441-8 - PubMed
    1. J Mol Biol. 1965 Feb;11:373-90 - PubMed
    1. Annu Rev Biochem. 1975;44:273-93 - PubMed
    1. Biochemistry. 1973 Feb 27;12(5):925-31 - PubMed

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