Successful pregnancies with concentrated frozen canine semen

Abstract

A method for concentrating canine semen prior to freezing was developed. Concentration was by centrifugation at 1470 g at room temperature, after which the majority of seminal fluid was removed. Motility, speed of progression, and morphology of sperm were not affected by concentration. Subsequent artificial insemination using concentrated semen resulted in a conception rate of 92%. The offspring were normal and healthy. The mean litter size (6.7) and birth weight (256.3 g) were comparable to those reported by a commercial supplier (6.2 and 255.7 g) of the same breed (beagle).