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. 1977 Dec;4(12):4077-89.
doi: 10.1093/nar/4.12.4077.

Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin

L Klevan, D M Crothers
Free PMC article

Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin

L Klevan et al. Nucleic Acids Res. 1977 Dec.
Free PMC article

Abstract

Calf thymus chromatin, depleted in histone H1, was digested with micrococcal nuclease and fractionated by column chromatography. 140 base pair nucleosome core particles were isolated along with an unusual particle containing 2 histone octamers and 240 base pairs of DNA. Evidence is presented that the spacer DNA region is absent from these modified dinucleosomes, which appear as stable products of the digestion process. The physical properties of both particles are presented along with brief speculation on their possible origin and function.

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References

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