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. 1977 Dec;4(12):4211-21.
doi: 10.1093/nar/4.12.4211.

Specificity of Hpa II and Hae III DNA methylases

M B Mann, H O Smith
Free PMC article

Specificity of Hpa II and Hae III DNA methylases

M B Mann et al. Nucleic Acids Res. 1977 Dec.
Free PMC article

Abstract

The methylases M.HaeIII and M.HpaII recognize the tetranucleotide sequences [Formula: see text] and [Formula: see text] respectively, in DNA, and transfer a methyl group from S-adenosylmethionine to the 5-position of cytosine on each strand as indicated by the asterisks. Restriction endonuclease R.HaeIII does not cleave the methylated sequence [Formula: see text] but can cleave [Formula: see text] in which methylation is introduced on the unnatural external cytosine positions. Similarly, R.HpaII does not cleave [Formula: see text] but can cleave [Formula: see text].Images

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References

    1. Cell. 1976 Jun;8(2):163-82 - PubMed
    1. Nucleic Acids Res. 1977 Feb;4(2):353-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 - PubMed
    1. Gene. 1976;1(1):81-92 - PubMed
    1. Nucleic Acids Res. 1976 Jul;3(7):1747-60 - PubMed

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