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. 1977 Dec;4(12):4391-410.
doi: 10.1093/nar/4.12.4391.

Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide

M J Gait, R C Sheppard
Free PMC article

Rapid synthesis of oligodeoxyribonucleotides. II. Machine-aided solid-phase syntheses of two nonanucleotides and an octanucleotide

M J Gait et al. Nucleic Acids Res. 1977 Dec.
Free PMC article

Abstract

Preparation of the two nonanucleotides, d(pG-G-A-G-G-A-G-A-A) and d(pT-T-C-T-G-T-T-G-A), and the octanucleotide, d(pT-G-G-C-C-C-A-T) is described by a rapid solid-phase method on a polyamide support. The syntheses were carried out using a modified solid-phase peptide synthesiser and required one day for each nucleotide addition. A microparticulate anion-exchanger, Partisil 10SAX, was used for the efficient fractionation of synthetic, base-protected oligonucleotides.

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References

    1. Biochemistry. 1963 Jul-Aug;2:697-702 - PubMed
    1. J Mol Biol. 1977 Jul;114(1):93-117 - PubMed
    1. Nucleic Acids Res. 1977 Feb;4(2):353-71 - PubMed
    1. Nucleic Acids Res. 1977 Apr;4(4):1123-34 - PubMed
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