D-glucose: preferential binding to brush borders disrupted with tris(hydroxymethyl)aminomethane

Abstract

The actively transported sugar D-glucose binds to brush borders disrupted with tris(hydroxymethyl)aminomethane in preference to D-mannose and L-glucose, which are not actively transported. This preferential binding of D-glucose is not dependent on either added Na(+) or adenosine triphosphatase activity stimulated by Na(+) with K(+) and Mg(2+), but it is temperature-dependent and is completely inhibited by 0.1 millimolar phlorizin and 1 millimolar mercuric chloride.