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. 1967 Apr 14;156(3772):256-7.
doi: 10.1126/science.156.3772.256.

Artifact produced in disc electrophoresis by ammonium persulfate

J M Brewer

Artifact produced in disc electrophoresis by ammonium persulfate

J M Brewer. Science. .

Abstract

Ammonium persulfate, a common polymerizing agent for acrylamide gels, can inactivate yeast enolase and produce increased electrophoretic heterogeneity during disc electrophoresis in gels containing 8M urea. The use of riboflavin and light for polymerization or thioglycolate for removal of the persulfate are feasible alternatives.

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