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. 1967 May;103(2):480-2.
doi: 10.1042/bj1030480.

A simple method for the isolation of adrenal chromaffin granules on a large scale

A D Smith, H Winkler

A simple method for the isolation of adrenal chromaffin granules on a large scale

A D Smith et al. Biochem J. 1967 May.

Abstract

Highly purified chromaffin granules can be obtained from homogenates of either ox, pig, horse or rat adrenal medullae by ultracentrifugation of the large-granule fraction layered on 1.6m-sucrose solution, by using angle-head rotors. The chromaffin granules are obtained as a pink sediment that is only slightly contaminated by mitochondria and lysosomes.

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