Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Aug;30(4):269-77.
doi: 10.1071/bi9770269.

Isolation of toxic metabolites of Phomopsis leptostromiformis responsible for lupinosis

C C Culvenor, A B Beck, M Clarke, P A Cockrum, J A Edgar, J L Frahn, M V Jago, G W Lanigan, A L Payne, J E Peterson, D S Petterson, L W Smith, R R White

Isolation of toxic metabolites of Phomopsis leptostromiformis responsible for lupinosis

C C Culvenor et al. Aust J Biol Sci. 1977 Aug.

Abstract

Two metabolites of P. leptostromiformis (phomopsins A and B) have been isolated as a crystalline mixture from a culture of the fungus on lupin seed. The mixture has been shown to be capable of inducing lupinosis in sheep and in young rats. Key steps in the isolation were the transfer of the phomopsins from crude aqueous solution to tetrahydrofuran and chromatography on macroreticular polystyrene resin. The bioassays used in monitoring fractions were based on inhibition of cell cultures and the mitosis-arresting effect of the metabolites on liver cells in vivo.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles