doi: 10.1083/jcb.34.2.627.
A chromatographic and spectrophotometric study of the products of the reaction of osmium tetroxide with unsaturated lipids
- PMID: 6035650
- PMCID: PMC2107326
- DOI: 10.1083/jcb.34.2.627
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A chromatographic and spectrophotometric study of the products of the reaction of osmium tetroxide with unsaturated lipids
J Cell Biol.
1967 Aug.
Abstract
Thin films of methyl oleate, oleic acid, and di-octadecenoyl phosphatidylcholine were reacted with a 2% solution of OsO(4) in water for 1 hr at 0 degrees . As controls, methyl 9,10-dihydroxystearate and 9,10-dihydroxystearic acid were reacted with OsO(4) in 0.25 N NaOH in methanol for 1 hr at room temperature. The reaction products were isolated, purified, and analyzed by thin-layer chromatography, gas-liquid chromatography, and infrared and visible spectroscopy. In all cases, the products were identified as diesters of osmic acid in which two molecules of fatty acids are linked through 1 molecule of osmic acid.
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