doi: 10.1007/BF01551817.
A procedure for the fusion of cells in suspension by means of polyethylene glycol
- PMID: 605382
- DOI: 10.1007/BF01551817
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A procedure for the fusion of cells in suspension by means of polyethylene glycol
Somatic Cell Genet.
1977 Mar.
Abstract
The nuclei of chick and human fibroblasts are readily distinguishable in Leishman stained heterokaryons. The use of these two types of cell has allowed development of a method for fusion in suspension based on the use of polyethylene glycol (PEG) of MW 1000 in Dulbecco's medium containing 15% dimethylsulfoxide (DMSO).
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