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. 1977 Dec;123(4):389-91.
doi: 10.1620/tjem.123.389.

Phytohemagglutinin-induced cytotoxic action of normal lymphocytes on cells in tissue culture using 14C-leucine incorporation

R Nemoto, K Ishikawa, T Kato
Free article

Phytohemagglutinin-induced cytotoxic action of normal lymphocytes on cells in tissue culture using 14C-leucine incorporation

R Nemoto et al. Tohoku J Exp Med. 1977 Dec.
Free article

Abstract

Stimulation of normal peripheral blood lymphocytes (PBL) by phytohemagglutinin (PHA) and production of PHA-induced cytotoxicity were investigated. PBL transformation stimulated by PHA was measured by 3H-thymidine incorporation, and the cytotoxicity was assayed by measuring the reduction of 14C-leucine incorporation in to target cells after interaction with PHA-treated PBL. The cytotoxicity of PHA-treated PBL showed dose response curves which corresponded with that of PBL transformation. Reduced 14C-leucine incorporation of target cells caused by cytotoxic PBL would be a reliable measure of immune reactions.

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