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. 1967 Oct;105(1):35-8.
doi: 10.1042/bj1050035.

Purification of an acid alpha-glucosidase by dextran-gel filtration

F Auricchio, C B Bruni

Purification of an acid alpha-glucosidase by dextran-gel filtration

F Auricchio et al. Biochem J. 1967 Oct.

Abstract

1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human kidney. 2. The acid alpha-glucosidases of both tissues show very similar pH optima when tested with maltose or glycogen as substrate.

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