Mode of action of antibiotic U-24,544

Abstract

Antibiotic U-24,544, a new antibacterial agent, was found to be an effective uncoupler of phosphorylation associated with the oxidation of glutamate and succinate in rat liver mitochondria. Respiration was inhibited during glutamate oxidation but not during succinate oxidation. In a medium deficient in inorganic phosphate, the agent showed slight stimulation of mitochondrial glutamate oxidation. Mitochondrial swelling induced by inorganic phosphate was suppressed. The antibiotic inhibited protein, nucleic acid, and cell wall synthesis in Mycobacterium avium cells nearly equally without a predominant inhibition of any one of these macromolecular biosynthetic processes. Nucleic acid and polypeptide synthesis remained unaffected, but respiration was inhibited in cell-free bacterial systems. It was thus concluded that the antibiotic interfered primarily with the cellular energy-generating processes.