Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase
- PMID: 60740
- PMCID: PMC343006
- DOI: 10.1093/nar/3.6.1473
Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase
Abstract
The effects of Mg++, Mn++, and KCl addition, individually and in combination, on the rate of DNA- and RNA-primed DNA synthesis by avian myeloblastosis virus DNA polymerase (reverse transcriptase) using a variety of natural and synthetic template-primer combinations were examined. Optimal divalent cation concentrations were found to vary by as much as 10-fold depending upon the template-primer used to direct synthesis. Addition of KCl to reaction mixtures containing optimal divalent cation concentrations produced stimulation or inhibition of DNA synthesis which was also template-specific. DNA synthesis on the modified template poly (2'-0-methylcytidylate) was uniquely stimulated by combinations of divalent cations. With Mg++ as divalent cation, deviations from classical Michaelis-Menten kinetics of substrate saturation were observed with all template-primers tested.
Similar articles
-
Spermine stimulates RNA-dependent reverse transcriptase activity.Biochem Int. 1989 Jul;19(1):67-76. Biochem Int. 1989. PMID: 2476130
-
Mechanism of interaction of avian myeloblastosis virus reverse transcriptase with avian myeloblastosis virus RNA.J Virol. 1976 Apr;18(1):26-33. doi: 10.1128/JVI.18.1.26-33.1976. J Virol. 1976. PMID: 56463 Free PMC article.
-
Utilization of mismatched initiator termini by avian myeloblastosis virus DNA polymerase.Biochim Biophys Acta. 1979 Dec 17;565(2):225-30. doi: 10.1016/0005-2787(79)90200-4. Biochim Biophys Acta. 1979. PMID: 92999
-
[DNA synthesis on the heterogeneous nuclear RNA template catalysed by DNA polymerase of avian myeloblastosis virus].Mol Biol (Mosk). 1975 Sep-Oct;9(5):768-74. Mol Biol (Mosk). 1975. PMID: 55956 Russian.
-
Reverse transcriptase.CRC Crit Rev Biochem. 1975 Dec;3(3):289-347. doi: 10.3109/10409237509105454. CRC Crit Rev Biochem. 1975. PMID: 56254 Review. No abstract available.
Cited by
-
Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification.Nucleic Acids Res. 2008 Sep;36(15):e92. doi: 10.1093/nar/gkn420. Epub 2008 Jul 4. Nucleic Acids Res. 2008. PMID: 18603591 Free PMC article.
-
Reverse transcriptase-associated RNase H activity. II. Inhibition by natural and synthetic RNA.J Virol. 1978 Sep;27(3):576-81. doi: 10.1128/JVI.27.3.576-581.1978. J Virol. 1978. PMID: 81313 Free PMC article.
-
On the early emergence of reverse transcription: theoretical basis and experimental evidence.J Mol Evol. 1992 Dec;35(6):524-36. doi: 10.1007/BF00160213. J Mol Evol. 1992. PMID: 1282161
-
The effect of magnesium and manganese ions on the structure and template activity for reverse transcriptase of polyribocytidylate and its 2'-0-methyl derivative.Nucleic Acids Res. 1977 Oct;4(10):3589-97. doi: 10.1093/nar/4.10.3589. Nucleic Acids Res. 1977. PMID: 73165 Free PMC article.
-
DNA endonucleases associated with the avian myeloblastosis virus DNA polymerase.Proc Natl Acad Sci U S A. 1979 Jun;76(6):2659-63. doi: 10.1073/pnas.76.6.2659. Proc Natl Acad Sci U S A. 1979. PMID: 223153 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
