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. 1984;43(11):1317-23.

The detection of distinct epitopes of human alpha-fetoprotein (AFP) by solid phase radioimmunoassay using hybridoma culture fluid

B Micheel, U Karsten, H Fiebach, T Porstmann, J Denner
  • PMID: 6085269

The detection of distinct epitopes of human alpha-fetoprotein (AFP) by solid phase radioimmunoassay using hybridoma culture fluid

B Micheel et al. Biomed Biochim Acta. 1984.

Abstract

A solid phase radioimmunoassay was developed for the epitope analysis of human alpha-fetoprotein (AFP) using hybridoma culture fluids. The following incubation sequence was performed: anti-mouse Ig, hybridoma culture fluid, normal mouse serum, and a mixture of 125I-labeled AFP and hybridoma culture fluid. Seven epitopes were detected by monoclonal antibodies produced by 15 presumably independent hybridoma clones.

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