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. 1984 Jul 25;259(14):9199-201.

Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins

K Mizuuchi, M Mizuuchi, M H O'Dea, M Gellert
  • PMID: 6086626
Free article

Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins

K Mizuuchi et al. J Biol Chem. .
Free article

Abstract

We have transferred the Escherichia coli gyrA and gyrB genes onto plasmids that allow the overproduction of the DNA gyrase A and B proteins and have designed relatively simple purification procedures for both proteins. The pure proteins are obtained in good yield; from 2 liters of culture (12 g of cells), one can recover 25 mg of GyrA or 3 mg of GyrB protein.

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