Restriction endonuclease analysis of Campylobacter strains with particular reference to Campylobacter fetus ss. fetus

Abstract

Forty-three strains of Campylobacter fetus ss. fetus isolated from sheep abortions in New Zealand, and reference strains of C. fetus ss. fetus (four), C. fetus ss. venerealis (two), C. jejuni (one) and C. coli (one) were examined by restriction endonuclease analysis with the enzymes BstE II and Xho I. DNA fragment patterns of C. fetus, C. jejuni and C. coli differed strikingly from each other, but there were many similarities in the patterns obtained for all strains of C. fetus ss. fetus (47) and C. fetus ss. venerealis (three). The 43 local strains of C. fetus ss. fetus were clearly divided into four types. Two of these types produced patterns nearly identical with those of overseas reference strains.