doi: 10.1093/nar/12.13.5165.
Alteration of apparent restriction endonuclease recognition specificities by DNA methylases
- PMID: 6087274
- PMCID: PMC318911
- DOI: 10.1093/nar/12.13.5165
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Alteration of apparent restriction endonuclease recognition specificities by DNA methylases
Nucleic Acids Res.
.
Free PMC article
Abstract
An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I in Adeno-2 DNA and for Acc I and Hinc II in pBR322 DNA by specifically methylating all but one recognition sequence.
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