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. 1984 Jul 11;12(13):5165-73.
doi: 10.1093/nar/12.13.5165.

Alteration of apparent restriction endonuclease recognition specificities by DNA methylases

M Nelson, C Christ, I Schildkraut
Free PMC article

Alteration of apparent restriction endonuclease recognition specificities by DNA methylases

M Nelson et al. Nucleic Acids Res. .
Free PMC article

Abstract

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I in Adeno-2 DNA and for Acc I and Hinc II in pBR322 DNA by specifically methylating all but one recognition sequence.

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