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. 1984 Aug;81(15):4642-6.
doi: 10.1073/pnas.81.15.4642.

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

A J Brake, J P Merryweather, D G Coit, U A Heberlein, F R Masiarz, G T Mullenbach, M S Urdea, P Valenzuela, P J Barr

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae

A J Brake et al. Proc Natl Acad Sci U S A. 1984 Aug.

Abstract

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

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