Detection of rotavirus in human stools by using monoclonal antibody

Abstract

A monoclonal antibody, 3F7, that reacts with the common rotavirus antigen on the sixth viral gene product was prepared. It was used in a direct monoclonal antibody radioimmunoassay (RIA) as a diagnostic reagent for detection, in 3.5 h, of rotavirus in human pediatric stool specimens. In the 177 samples tested, a concordance of 96% was seen between the monoclonal RIA and the well-established and commonly used commercially available Rotazyme test. Six discrepant specimens that were positive by monoclonal RIA but negative by Rotazyme were shown to be positive by either electron microscopy or confirmatory blocking immunoassay. A seventh discrepant specimen was positive by Rotazyme and negative by monoclonal RIA as well as by both direct and immune electron microscopy. The monoclonal RIA test appears to be highly sensitive and specific, and merits additional evaluation as a rapid, convenient diagnostic assay that can reduce currently encountered problems associated with diagnosing rotavirus infection by immunoassay.