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. 1984 May-Jun;18(3):599-606.

[Termination of DNA transcription by a poly(dA).poly(dT) fragment inserted into pBR325 plasmid]

[Article in Russian]
L Ia Denisova, S N Zagrebel'nyĭ, N M Pustoshilova, N I Putintseva, L I Puchkova
  • PMID: 6088967

[Termination of DNA transcription by a poly(dA).poly(dT) fragment inserted into pBR325 plasmid]

[Article in Russian]
L Ia Denisova et al. Mol Biol (Mosk). 1984 May-Jun.

Abstract

A recombinant DNA was constructed by inserting polynucleotide (dA).(dT) of 80-100 base pairs long into EcoRI site of the pBR325 plasmid DNA. Transcription of this DNA was studied in E. coli RNA polymerase system in vitro. Some transcripts obtained with the recombinant plasmid were shown to have poly(U) clusters at the 3'-ends. Obtained data indicate that poly(dA).poly(dT) sequence acts as a terminator of RNA synthesis. Orientation of this sequence in recombinant DNA was also established.

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