Secretion of human parathyroid hormone from rat pituitary cells infected with a recombinant retrovirus encoding preproparathyroid hormone
- PMID: 6089196
- PMCID: PMC391699
- DOI: 10.1073/pnas.81.17.5340
Secretion of human parathyroid hormone from rat pituitary cells infected with a recombinant retrovirus encoding preproparathyroid hormone
Abstract
In order to study the functions of precursors to secreted proteins, we expressed cloned DNA encoding human preproparathyroid hormone (preproPTH) in rat pituitary cells. We first constructed a recombinant plasmid containing human preproPTH cDNA and retroviral control signals. This recombinant plasmid was transfected into psi-2 cells, a packaging cell line that produces Moloney murine leukemia viral particles containing no retroviral RNA. The transfected psi-2 cells generated helper-free recombinant retrovirus encoding preproPTH, and this recombinant retrovirus was used to infect GH4 rat pituitary cells. Clonal lines of the infected GH4 cells contained copies of the recombinant provirus stably integrated via the long terminal repeats, and the expected RNA transcripts of proviral DNA accumulated in the cytoplasm, although no infectious virus was produced. The infected cells synthesized and processed preproPTH appropriately and secreted PTH in response to thyrotropin-releasing hormone, a secretagogue for GH4 cells. Use of recombinant retrovirus permits the introduction of DNA encoding normal and mutant secreted proteins into a number of cell types specialized for secretion. Analysis of the fate of the resultant proteins will help define the specific molecular interactions involved in transmembrane transport and processing of precursor proteins.
Similar articles
-
Gene encoding parathyroid hormone. Nucleotide sequence of the rat gene and deduced amino acid sequence of rat preproparathyroid hormone.J Biol Chem. 1984 Mar 10;259(5):3320-9. J Biol Chem. 1984. PMID: 6321505
-
Cloning and sequence analysis of the complementary DNA for feline preproparathyroid hormone.Am J Vet Res. 2002 Feb;63(2):194-7. doi: 10.2460/ajvr.2002.63.194. Am J Vet Res. 2002. PMID: 11843117
-
Nucleotide sequence of cloned cDNAs encoding chicken preproparathyroid hormone.J Bone Miner Res. 1988 Dec;3(6):689-98. doi: 10.1002/jbmr.5650030615. J Bone Miner Res. 1988. PMID: 3251402
-
Nucleotide sequence of cloned cDNAs encoding human preproparathyroid hormone.Proc Natl Acad Sci U S A. 1981 Dec;78(12):7365-9. doi: 10.1073/pnas.78.12.7365. Proc Natl Acad Sci U S A. 1981. PMID: 6950381 Free PMC article.
-
Molecular biology of parathyroid hormone.CRC Crit Rev Biochem. 1986;19(4):353-79. doi: 10.3109/10409238609082545. CRC Crit Rev Biochem. 1986. PMID: 3516570 Review.
Cited by
-
Gene transfer into the nervous system.Mol Neurobiol. 1987 Winter;1(4):339-71. doi: 10.1007/BF02935741. Mol Neurobiol. 1987. PMID: 3077063 Review. No abstract available.
-
Expression of human adenosine deaminase using a transmissable murine retrovirus vector system.Proc Natl Acad Sci U S A. 1985 Feb;82(3):703-7. doi: 10.1073/pnas.82.3.703. Proc Natl Acad Sci U S A. 1985. PMID: 3856223 Free PMC article.
-
Posttranslational processing of endogenous and of baculovirus-expressed human gastrin-releasing peptide precursor.Mol Cell Biol. 1988 Aug;8(8):3129-35. doi: 10.1128/mcb.8.8.3129-3135.1988. Mol Cell Biol. 1988. PMID: 3211139 Free PMC article.
-
Expression of the human proenkephalin gene in mouse pituitary cells: accurate and efficient mRNA production and proteolytic processing.EMBO J. 1985 Dec 1;4(12):3115-22. doi: 10.1002/j.1460-2075.1985.tb04053.x. EMBO J. 1985. PMID: 3004933 Free PMC article.
-
A bicistronic Epstein-Barr virus mRNA encodes two nuclear proteins in latently infected, growth-transformed lymphocytes.J Virol. 1987 Apr;61(4):945-54. doi: 10.1128/JVI.61.4.945-954.1987. J Virol. 1987. PMID: 3029429 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical