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. 1984 Aug 15;222(1):125-30.
doi: 10.1042/bj2220125.

Effects of phosphorylation on the kinetic properties of rat liver fructose-1,6-bisphosphatase

D W Meek, H G Nimmo

Effects of phosphorylation on the kinetic properties of rat liver fructose-1,6-bisphosphatase

D W Meek et al. Biochem J. .

Abstract

A new purification procedure for rat liver fructose-1,6-bisphosphatase that involves use of Procion Red-Sepharose is described. The purified enzyme was homogeneous, had a subunit Mr of 40 000-41 000 and seemed to be undegraded. The enzyme could be phosphorylated by cyclic AMP-dependent protein kinase with a stoicheiometry of one per subunit. Phosphorylation caused a 2-fold decrease in the Km of the enzyme for fructose 1,6-bisphosphate, but did not affect its allosteric responses to AMP, Mg2+ and fructose 2,6-bisphosphate.

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References

    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Biochim Biophys Acta. 1981 Dec 15;662(2):265-70 - PubMed
    1. Adv Exp Med Biol. 1974;42(0):123-33 - PubMed
    1. Adv Enzymol Relat Areas Mol Biol. 1975;42:193-226 - PubMed
    1. Anal Biochem. 1975 May 26;66(1):253-8 - PubMed

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