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. 1984;90(2):128-32.
doi: 10.1007/BF00292449.

Restriction enzyme banding of mouse metaphase chromosomes

M Kaelbling, D A Miller, O J Miller

Restriction enzyme banding of mouse metaphase chromosomes

M Kaelbling et al. Chromosoma. 1984.

Abstract

Fixed metaphase chromosomes from mouse strain RIII embryos or A9 cells were treated with a restriction endonuclease, followed by Giemsa staining. Alu I, Hinf I, or Mbo I treatment produced a C-band pattern, and Eco RII or Hae III produced a G-band plus C-band pattern. Ava II and Bst NI each produced a G-band pattern, but on most chromosomes only a small segment of each C-band, adjacent to the centromere, was stained. These tiny residual C-bands may contain a minor satellite located adjacent to the major satellite clusters.

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