Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Oct;160(1):15-21.
doi: 10.1128/jb.160.1.15-21.1984.

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation

M Y Yang, E Ferrari, D J Henner

Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation

M Y Yang et al. J Bacteriol. 1984 Oct.

Abstract

The neutral protease gene of Bacillus subtilis has been cloned, and its nucleotide sequence has been determined. The cloned gene was used to create an in vitro-derived deletion mutation, which was used to replace the wild-type copy of the gene. This deletion, in combination with a deletion of the alkaline protease gene, completely abolished protease production. The loss of the proteases had no detectable effect on growth, morphology, or sporulation.

PubMed Disclaimer

References

    1. J Bacteriol. 1980 Mar;141(3):1199-208 - PubMed
    1. J Bacteriol. 1961 May;81(5):741-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 May;76(5):2232-5 - PubMed
    1. J Bacteriol. 1983 Jun;154(3):1513-5 - PubMed
    1. Biochimie. 1971;53(10):1059-66 - PubMed

Associated data

LinkOut - more resources