Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jul;12(3):273-86.
doi: 10.1016/0166-6851(84)90084-7.

Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species

J M Ntambi, J C Marini, J D Bangs, S L Hajduk, H E Jimenez, P A Kitchin, V A Klein, K A Ryan, P T Englund

Presence of a bent helix in fragments of kinetoplast DNA minicircles from several trypanosomatid species

J M Ntambi et al. Mol Biochem Parasitol. 1984 Jul.

Abstract

Some restriction fragments of kinetoplast minicircles from several trypanosomatid species (Leishmania tarentolae, Trypanosoma brucei, T. equiperdum, Herpetomonas muscarum, Crithidia fasciculata, but not T. cruzi) migrate anomalously on polyacrylamide gels. This behavior is probably due to a natural curvature of the helix. Bent helices appear to be a common property of kinetoplast minicircles, and may be important for minicircle function. In the case of T. equiperdum, we present evidence that each minicircle has a single bent region which resides in or near the 'conserved sequence.'

PubMed Disclaimer

Publication types

LinkOut - more resources