Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Sep 11;12(17):6663-71.
doi: 10.1093/nar/12.17.6663.

Codon usage can affect efficiency of translation of genes in Escherichia coli

M Robinson, R Lilley, S Little, J S Emtage, G Yarranton, P Stephens, A Millican, M Eaton, G Humphreys
Free PMC article

Codon usage can affect efficiency of translation of genes in Escherichia coli

M Robinson et al. Nucleic Acids Res. .
Free PMC article

Abstract

By inserting synthetic oligonucleotides into a highly expressed gene in E. coli it has been shown that unfavourable codon usage can reduce the maximum translation rate of a protein. However, in the case of the codon used (AGG), a significant effect on translation was only seen at very high transcription rates from a gene containing multiple copies of the unfavourable codon.

PubMed Disclaimer

References

    1. J Mol Biol. 1966 Aug;19(2):548-55 - PubMed
    1. J Mol Biol. 1969 May 14;41(3):459-72 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Mol Biol. 1971 Nov 28;62(1):179-88 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed