doi: 10.1093/nar/12.17.6701.
RsrII--a novel restriction endonuclease with a heptanucleotide recognition site
- PMID: 6091034
- PMCID: PMC320110
- DOI: 10.1093/nar/12.17.6701
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RsrII--a novel restriction endonuclease with a heptanucleotide recognition site
Nucleic Acids Res.
.
Free PMC article
Abstract
A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence: (sequence; see test) By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.
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