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. 1984 Sep 11;12(17):6701-8.
doi: 10.1093/nar/12.17.6701.

RsrII--a novel restriction endonuclease with a heptanucleotide recognition site

C D O'Connor, E Metcalf, C J Wrighton, T J Harris, J R Saunders
Free PMC article

RsrII--a novel restriction endonuclease with a heptanucleotide recognition site

C D O'Connor et al. Nucleic Acids Res. .
Free PMC article

Abstract

A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence: (sequence; see test) By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.

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