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. 1984 Sep 11;12(17):6887-900.
doi: 10.1093/nar/12.17.6887.

Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family

T Blankenstein, G Zoebelein, U Krawinkel
Free PMC article

Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family

T Blankenstein et al. Nucleic Acids Res. .
Free PMC article

Abstract

A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.

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References

    1. Science. 1977 Apr 8;196(4286):180-2 - PubMed
    1. J Mol Biol. 1977 Jun 15;113(1):237-51 - PubMed
    1. Eur J Immunol. 1978 Jun;8(6):393-400 - PubMed
    1. Cell. 1978 Dec;15(4):1133-44 - PubMed
    1. Nature. 1979 Jul 26;280(5720):288-94 - PubMed

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