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. 1984 Jul-Aug;29(1-2):87-92.
doi: 10.1016/0378-1119(84)90169-0.

Sequencing of coronavirus IBV genomic RNA: a 195-base open reading frame encoded by mRNA B

M E Boursnell, T D Brown

Sequencing of coronavirus IBV genomic RNA: a 195-base open reading frame encoded by mRNA B

M E Boursnell et al. Gene. 1984 Jul-Aug.

Abstract

DNA sequencing of genomic cDNA clones of avian infectious bronchitis virus (IBV) has been carried out. 770 bases have been determined which include genomic sequences spanning the 5' termini of the two smallest mRNAs of the 3'-coterminal "nested" set: mRNA A and mRNA B. This region contains the complete coding sequences for mRNA B which are additional to those present in mRNA A. Two open reading frames are present, predicting proteins of Mrs 7500 and 9500.

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References

    1. Armstrong J., Smeekens S., Rottier P. Sequence of the nucleocapsid gene from murine coronavirus MHV-A59. Nucl. Acids Res. 1983;11:883–891. - PMC - PubMed
    1. Baric S., Stohlman S., Lai M.M.C. Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader sequences on nascent chains. J. Virol. 1983;48:633–640. - PMC - PubMed
    1. Brown T.D.K., Boursnell M.E.G. Avian infectious bronchitis virus genomic RNA contains sequence homologies at the intergenic boundaries. Virus Res. 1984;1:15–24.
    1. Cavanagh D. Structural polypeptides of coronavirus IBV. J. Gen. Virol. 1981;53:93–103. - PubMed
    1. Holmes D.S., Quigley M. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 1981;114:193–197. - PubMed

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