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. 1984 Aug;4(8):1641-3.
doi: 10.1128/mcb.4.8.1641-1643.1984.

Short-term, high-efficiency expression of transfected DNA

D J Sussman, G Milman

Short-term, high-efficiency expression of transfected DNA

D J Sussman et al. Mol Cell Biol. 1984 Aug.

Abstract

We have achieved high-efficiency uptake and expression of foreign DNA in mouse Ltk- cells by modifying the DEAE-dextran-mediated transfection method of McCutchan and Pagano (J. Natl. Cancer Inst. 42:351-357, 1968) to include an initial incubation at elevated pH followed by a shock treatment with dimethyl sulfoxide. Up to 80% of mouse Ltk- cells transfected with the herpes simplex virus thymidine kinase gene expressed thymidine kinase as measured by autoradiography.

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References

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