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. 1984 Sep;4(9):1695-705.
doi: 10.1128/mcb.4.9.1695-1705.1984.

New human transforming genes detected by a tumorigenicity assay

O Fasano, D Birnbaum, L Edlund, J Fogh, M Wigler

New human transforming genes detected by a tumorigenicity assay

O Fasano et al. Mol Cell Biol. 1984 Sep.

Abstract

We have developed a sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice. The assay differs substantially from the NIH3T3 focus assay. Using it, we have detected the transfer of three transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which we have called mcf2 and mcf3, do not appear to be related to known oncogenes. We cannot detect their transfer by using the NIH3T3 focus assay. We do not yet know whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells.

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References

    1. J Virol. 1969 Nov;4(5):549-53 - PubMed
    1. Virology. 1973 Apr;52(2):456-67 - PubMed
    1. J Natl Cancer Inst. 1973 Nov;51(5):1409-16 - PubMed
    1. J Bacteriol. 1975 Jan;121(1):354-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 - PubMed

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