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. 1984 Nov;81(21):6681-5.
doi: 10.1073/pnas.81.21.6681.

Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies

A H Ross, P Grob, M Bothwell, D E Elder, C S Ernst, N Marano, B F Ghrist, C C Slemp, M Herlyn, B Atkinson, et al.

Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies

A H Ross et al. Proc Natl Acad Sci U S A. 1984 Nov.

Abstract

The nerve growth factor (NGF) receptor was characterized by using a new series of anti-receptor monoclonal antibodies (MAbs). These MAbs (i) showed significantly greater reactivity with a melanoma cell line expressing higher levels of NGF receptor, (ii) inhibited the binding of 125I-labeled NGF to its receptor, and (iii) immunoprecipitated both metabolically labeled and 125I-labeled NGF affinity-labeled receptor. These experiments defined the receptor as a 75-kDa cell-surface protein. The NGF receptor was visualized by immunoperoxidase staining in tissue sections of human nevi, melanomas, neurofibromas, a pheochromocytoma, and peripheral nerves. Uniform staining of the cytoplasm suggests that, in addition to cell-surface NGF receptors, there is a population of intracellular receptors.

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References

    1. Physiol Rev. 1968 Jul;48(3):534-69 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3556-60 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Feb;74(2):565-9 - PubMed
    1. J Biol Chem. 1979 Jul 10;254(13):5972-82 - PubMed
    1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4188-92 - PubMed

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