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. 1984 Nov 12;12(21):8085-96.
doi: 10.1093/nar/12.21.8085.

Identification, physical map location and sequence of the denV gene from bacteriophage T4

K Valerie, E E Henderson, J K deRiel
Free PMC article

Identification, physical map location and sequence of the denV gene from bacteriophage T4

K Valerie et al. Nucleic Acids Res. .
Free PMC article

Abstract

The denV gene from bacteriophage T4, which codes for endonuclease V, a small DNA repair enzyme, has been cloned and identified by an approach combining DNA sequencing and genetics, independent of the phenotypic effect of the cloned gene. Appropriate DenV+ and DenV- deletion mutants were mapped physically to define precisely a region encompassing the denV gene. This region was sequenced in order to identify a protein-coding sequence of the correct size for the denV gene (400-500 bp). Finally, identification was confirmed by sequencing the corresponding fragments cloned from four genetically and phenotypically well-characterized denV mutants. The denV gene is located at 64 kb on the T4 genome, adjacent to the ipII gene, and codes for a basic protein of 138 amino acids with a deduced molecular weight of 16,078.

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