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. 1984 Dec;81(23):7392-6.
doi: 10.1073/pnas.81.23.7392.

Duplicated heavy metal control sequences of the mouse metallothionein-I gene

A D Carter, B K Felber, M J Walling, M F Jubier, C J Schmidt, D H Hamer

Duplicated heavy metal control sequences of the mouse metallothionein-I gene

A D Carter et al. Proc Natl Acad Sci U S A. 1984 Dec.

Abstract

We present evidence that two distinct regions of the DNA upstream from the mouse metallothionein-I gene contain metal-responsive regulatory sites. This result was obtained by analyzing a systematic series of deletion, insertion, duplication, and clustered point mutations introduced into cultured cells on a simian virus 40 plasmid vector. The two upstream regions contain a duplicated evolutionarily conserved DNA sequence. While either upstream region is sufficient to confer heavy metal responsiveness, both are required to give maximal levels of induced transcription.

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