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. 1984 Dec;81(23):7500-4.
doi: 10.1073/pnas.81.23.7500.

Enhanced recombination between lambda plac5 and F42lac: identification of cis- and trans-acting factors

H S Seifert, R D Porter

Enhanced recombination between lambda plac5 and F42lac: identification of cis- and trans-acting factors

H S Seifert et al. Proc Natl Acad Sci U S A. 1984 Dec.

Abstract

Enhanced transductional recombination between specialized transducing phage lambda plac5 and plasmid F42lac depends on cis- and trans-acting factors. By constructing a series of recombinant molecules, the cis-acting site required for enhanced recombination has been identified as oriT, the origin of conjugational transfer of the F sex factor of Escherichia coli. The trans-acting factors are located in the promoter-proximal and the promoter-distal regions of the traY-to-traZ operon.

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