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. 1984 Sep:21 ( Pt 5):366-71.
doi: 10.1177/000456328402100505.

A spectrophotometric assay of phosphoribosyl pyrophosphate synthetase

J Braven, T R Hardwell, R Seddon, M Whittaker

A spectrophotometric assay of phosphoribosyl pyrophosphate synthetase

J Braven et al. Ann Clin Biochem. 1984 Sep.

Abstract

A simple spectrophotometric assay of phosphoribosyl pyrophosphate synthetase from human erythrocytes is described. The kinetic properties of the enzyme have been determined and are in good agreement with published results. Conditions for storing haemolysates at -20 degrees C are described. The method is suitable for semi-automation.

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