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Comparative Study
. 1984 Oct;30(1-3):257-60.
doi: 10.1016/0378-1119(84)90130-6.

High-copy-number derivatives of the plasmid cloning vector pBR322

I Boros, G Pósfai, P Venetianer
Comparative Study

High-copy-number derivatives of the plasmid cloning vector pBR322

I Boros et al. Gene. 1984 Oct.

Abstract

A stable copy-number mutant of a pBR322-derived plasmid was isolated. The mutation was found to be a single G----T transversion located near the 3' end of a DNA segment coding for the regulatory RNA I. The resulting copy number for this plasmid is approx. 1000 per cell or 65% of total cellular DNA. Several cloning vectors have been constructed from this copy-number mutant and their practical application is discussed.

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